Fetal bovine serum main function and preservation method - Database & Sql Blog Articles

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Main functions of fetal calf serum:

1) Provide hormones that maintain cell growth

2) Acid-base buffer solution

3) Provide protease inhibitors to protect cells from harm when cells are passaged

4) Sources of factors required for cell attachment and spreading on plastic substrates

5) Provide binding protein, bind or regulate the vitality of the binding substances of vitamins, lipids, metals and other hormones; combine with toxic metals and pyrogens to detoxify.

Fetal bovine serum standard parameters:

1) Endotoxin: The standard is not higher than 5EU/ml, and the high content indicates that it has been contaminated.

2) Total protein content: fetal bovine serum is generally in the range of 30-40mg/ml

3) Hemoglobin: the standard is not higher than 20mg/dl

Serum type:

1) Fetal bovine serum (FBS): Eight-month-old fetal bovine heart puncture blood separation serum

2) Newborn bovine serum (NBCS): venous blood collection of newborn cattle at 12-24h

3) Calf serum (CS): Three-month-old calf arterial blood collection serum

Fetal bovine serum grade: New Zealand>Australia>North America>The blood supply to the former domestic market in South America is mainly China, South America and Australia.

Serum preservation method: It is recommended to store at -20 °C. Do not use more than one month at 4 °C; if you can't use one bottle at a time, pack it into a sterile container and freeze it to avoid repeated freezing and thawing. Thawing: After taking the serum out of the freezer, it is placed in a refrigerator at 2-8 °C to dissolve slowly. When it is dissolved, it should be shaken regularly.