How can we avoid the precipitation in serum? -Huaqiang Electronic Network

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How can I avoid it?

What is the precipitation? First, we need to understand what is the main role of serum and serum refers to the removal of the light yellow transparent liquid separated from fibrinogen in plasma or the plasma in which the fiber white egg has been removed after blood coagulation. Its primary role is to provide essential nutrients, provide hormones and various growth factors, provide binding proteins, provide pro-contact and stretching factors to protect cells from mechanical damage, and provide some protection to cells in culture.

main effect

1 Provide basic nutrients: amino acids, vitamins, inorganic substances, lipids, nucleic acid derivatives, etc., which are essential for cell growth.

2 provide hormones and various growth factors: insulin, adrenocortical hormone (dexamethasone), steroid hormones (estradiol, testosterone, progesterone) and the like. Growth factors such as fibroblast growth factor, epidermal growth factor, platelet growth factor and the like.

3 provides a binding protein: the binding protein acts to carry important low molecular weight substances, such as albumin carrying vitamins, fats, and hormones, and transferrin carries iron. Binding proteins play an important role in cellular metabolism.

4 Provides pro-contact and stretching factors to protect cells from mechanical damage.

5 It has some protective effects on the cells in culture: some cells, such as endothelial cells and bone marrow-like cells, can release proteases, and the serum contains anti-protease components to neutralize. This effect was discovered by chance, and the purpose of using serum to stop the digestion of trypsin. Because trypsin has been widely used for digestion and passage of adherent cells. Serum proteins form the viscosity of serum and protect cells from mechanical damage, especially when suspended in suspension culture. Serum also contains trace elements and ions, which play an important role in metabolic detoxification, such as SeO3, selenium, etc.

The following operations can effectively prevent precipitation:

(1) The heat inactivation of serum is very likely to cause an increase in sediment, and this step can be skipped if it is not necessary.

(2) If you must do the heat inactivation of serum, be sure to remember the principle of 30 ° at 56 ° C, and shake it at any time. If the temperature is too high, too long a time or uneven shaking will result in an increase in sediment.

(3) When the serum is thawed, it is ready to shake evenly to make the temperature and composition uniform, which can effectively reduce the occurrence of sedimentation.

(4) When the serum is frozen and stored, it should be shaken regularly (be careful not to cause bubbles), so that the temperature and composition are uniform.

(5) Do not directly thaw from -20 °C to 37 °C. Because the temperature changes too much, it is easy to cause protein to coagulate and precipitate.

(6) Do not put the serum at 37 °C for too long, otherwise the serum will become turbid, and many of the more unstable components in the serum will be destroyed, thus affecting the quality of the serum.

I hope that these can help you effectively. Shanghai Jinma Experimental Equipment Co., Ltd. double guarantees the quality and price. You are welcome to inquire.