There are generally two antigen-antibody reactions in the elisa kit, ie, after adding the sample and adding the enzyme conjugate. The completion of the antigen-antibody reaction requires a certain temperature and time. This incubation process is called incubation, which is called incubation and may not be appropriate in an ELISA kit.
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ELISA is a solid phase immunoassay in which antigen and antibody binding occurs only on the surface of a solid phase. Taking the sandwich method of antibody coating as an example, the specimen added to the well of the plate does not have an equal opportunity to bind to the solid phase, and only the antigen in the solution closest to the pore wall is directly in contact with the antibody. . This is a process of gradual equilibrium, so it needs to be diffused to reach the end of the reaction. The same applies to the binding of the enzyme-labeled antibody added thereafter to the solid phase antigen. This is why ELISA reactions always require a certain amount of incubation.
The temperatures commonly used for incubation are 43 ° C, 37 ° C, room temperature and 4 ° C. 37 ° C is the usual incubation temperature in the laboratory, and is also the appropriate temperature for most antigen-antibody binding. When the ELISA method was established as the reaction kinetics study, the experiment showed that the two antigen-antibody reactions generally reached the peak at 37 ° C for 1 to 2 hours. In order to accelerate the reaction, the temperature of the reaction can be raised. Some tests are carried out at 43 ° C, but higher temperatures are not suitable. The antigen-antibody reaction was more thorough at 4 ° C, and the reaction was mostly allowed to stand overnight in the refrigerator in a radioimmunoassay to form the most precipitate. However, because it takes too long, it is generally not used in ELISA kits.
Insulation method In addition to the special ELISA instrument with special electric heating block, the water bath is generally used. The ELISA kit plate can be placed in the water bath box. The bottom of the ELISA plate should be attached to the water surface to make the temperature balance quickly. In order to avoid evaporation, the board should be covered, or the plate hole can be covered with plastic sealing paper or plastic wrap. At this time, the reaction plate can float on the water surface. If an incubator is used, the ELISA kit should be placed in a wet box. The wet box should be made of a material with good heat transfer such as metal, a wet gauze at the bottom of the box, and finally the ELISA plate should be placed on the wet gauze. The wet box should be pre-warmed in the incubator to the specified temperature, especially at lower temperatures. Whether it is water bath or wet box incubation, the reaction plates should not be stacked to ensure that the temperature of each plate can be quickly balanced. For the reaction at room temperature, the room temperature during the operation should be strictly limited to the specified range. The standard room temperature refers to 20 to 25 ° C, but the specific conditions can be controlled according to the requirements of the instructions. When incubated at room temperature, the ELISA kit can be placed flat on the console. It should be noted that the temperature and time of incubation should be as accurate as required. In order to ensure this, one person should not measure more than two plates at the same time.
Built-in 3.6V rechargeable Batteries ( . The purpose of the batteries is to store the total electricity and memory setting
Resetting
If an abnormal display appears or the buttons produce no response, the instrument must be reset. To do this,
press the RESET button.
Display Mode
Entire LCD can be displayed for about 1 minute and then it automatically gets into Model. To transfer from
one mode to the other, press the FUNCTION button.
Mode 1: Time/Watt/Cost Display Display duration(how long) this device connect to power source.LCD on first line shows 0:00 with first two figures mean minutes(2 figures will occur while occur at 10 min) and the rest shows seconds. After 60mins, it displays 0:00 again with first two numbers meas hour(2 figures will occur at 10hours)and the rest shows minutes. The rest can be done in the same manner which means after 24 hours, it will re-caculate. LCD on second line displays current power which ranges in 0.0W 〜 9999W. LCD on third line displays the current electricity costs which ranges in O.Ocost 〜 9999cost. It will keep on O.OOcost before setting rate without other figures.
Mode 2: Time/Cumulative electrical quantity Display Display duration(how long) this device connect to power source.
LCD on first line shows 0:00 with first two figures mean minutes(2 figures will occur while occur at 10 min) and the rest shows seconds. After 60mins, it displays 0:00 again with first two numbers meas hour(2 figures will occur at 10hours)and the rest shows minutes. The rest can be done in the same manner which
means after 24 hours, it will re-caculate. LCD on second line displays current cumulative electrical quantity which ranges in 0.000KWH 〜 9999KWH without other figures. LCD on third line displays"DAY"- "1 'Will be showed on numerical part(the other three figures will be showed at carry) which means it has cumulated electrical quantity for 24hours(one day). The rest can be done in the same manner untill the maximal cumulative time of 9999 days.
Mode 3: TimeA^bltage/Frequency Display LCD on first line displays the same as Mode 1 dones. LCD on second line displays current voltage supply (v) which ranges in 0.0V 〜 9999V .LCD on third line displays current frequency (HZ) which ranges in 0.0HZ 〜 9999Hz without other figures.
Mode 4: Time/Current/Power Factor Display LCD on first line displays the same as Mode 1 dones.LCD on second line displays load current which ranges in 0.0000A 〜 9999A. LCD on third line displays current power factor which ranges in 0.00PF 〜 LOOPF without other figures.
Mode 5:Time/Minimum Power Display LCD on first line displays the same as Mode 1 dones. LCD on
second line displays the minimum power which ranges in 0.0W 〜 9999W. LCD on third line displays character of "Lo" without other figures.
Mode 6: Time/Maximal Power Display LCD on first line displays the same as Mode 1 dones. LCD on second line displays the maximal power which ranges in 0.0W 〜 9999W. LCD on third line displays character of "Hi" without other figures.
Mode 7: Time/Price Display LCD on first line displays the same as Mode 1 dones. LCD on third line displays the cost which ranges in O.OOCOST/KWH 〜 99.99COST/KWH without other figures.
Overload Display: When the power socket connects the load over 3680W, LCD on second line displays the''OVERLOAD[ with booming noise to warn the users, (selectable choice)
Supplemental informations:
1: Except [OVERLOAD[ interface, LCD on first line display time in repitition within 24hours.
2: LCD on first line, second line or third line described in this intruction take section according to two black lines on LCD screen. Here it added for clarified purpose.
3. Mode 7 will directly occur while press down button "cost".
4. [UP"&"Down" are in no function under un-setting mode.
Setting Mode
1. Electricity price setting
After keeping COST button pressed lasting more than 3 seconds(LCD on third line display system defaults price, eg O.OOCOST/KWH ),the rendered content begins moving up and down which means that the device
has entered the setting mode. After that, press FUNCTION for swithing , then press "UP"and "DOWN" button again to set value which ranges in OO.OOCOST/KWH 〜 99.99COST/KWH. After setting all above, press COST to return to Mode7 or it will automatically return to Mode7 without any pressing after setting with data storage.
December 19, 2022